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2.????????????????????????????θ???????????????????????θ????????????????η?壬??????????????????????Ч??????????????????????????????????????????????糤?????????????????????????????壬?????С???????????????????????????????磬????м??С???????????????ζ????????????????θ?????????????????????

?????????Xi??n H?? C??o

?????????Hairyvein Agrimonia Herband Bud, Herb of Hairyvein Agrimonia

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????????????????Agrimonia pilosa Ledeb.

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????????????? ?????????????30-120cm?????????????????1??????????????????????????????????????賤??????????????????????????Σ?????Σ?????????????????????????????С??д?С2????????????????????С?3-4????2????????????3С???С??????????????????????????Σ???1.5-5cm????1-2.5cm???????????????????????Ш?Σ?????м??????????????????????????????浭????????????????????????????????????????????????2-3????????????????????????????1-5mm???????????????3???????????Σ?С????????????Σ?????????????????6-9mm?????5?????????Σ?????5??????Σ??????????5-15??????2???????????????????????Σ???????10????????????????????????????????????????????????????????7-8mm???????????3-4mm????????5-12???

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?????? ?????????????????????????????3???????????4???????????????????9???????10?????????????10???????11???????????????????????1.3m??裬???????о?30-40cm??1-2cm??????????????????????????????????????????1hm2???????15-22.5kg??????????25?棬????10-15d???硣????????????????????????У???????????????????2-3????????????????????????30cm??15cm????15cm????????1????????5cm?????????????????????????????棬??????????????95%?????

?????? ???3-5cm????硢???磬???????????磬???15cm??????15cm???硣??????????г???????????????????в????ε??????????????????????????????1hm2 1500-22500kg?????????????????????????????

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??????? ??峤50-100cm?????????????????????Σ????0.4-0.6cm?????????????????Σ??????????????????????????????н??????????????????????п??????????????????????????????????????????飻???д?С2????????????????????С????????С???????????λ?????Σ?????????Ш?Σ?????о??????2????????б???Ρ?????????[FS:PAGE]?????????0.6-0.9cm??????????????????????й???????5????????????????0.7-0.8cm?????0.3-0.4cm???????ζ???????????????????

??????? ?????????????????????????????Σ??±??????????????????????????????????????????????????????????????????????????????????????????1-4?????????Σ??????1-2??????????????????????????????????????68??m?????????Σ?????????????????????????9-50??m??

????????????????????????????(Agrimonolide)??????(???????????????????????(6.67%-13.71%))????????л???????????????????????????A(agrimol A)???????B(agrimol B)???????C(agrimol C)???????D(agrimol D)???????E(agrimol E)???????F(agrimol F)???????G(agrimol G)???????????C???????????к???????-7-D-(luteolin-7-D-glucoside)????????(cosmosin)?????????????????з?????????????????(agrimonin A,B,C)???????????????????????????????Ρ????????????????????8.9%??????????6.5%?????????16.4%?????????????????-7-??-???????(Luteolin-7-??-glucoside)???????-7-??-???????(Apigenin-7-??-glucoside)???????C??K????????????????(agrimophol)??????????????????????????????Ч??????к?????????(agrimonolide)???????(vamillic acid)??l-????????(l-??????????l-taxifolin)????????α???????????????-6-O-??-D-??????????????????????????(2S,3S)-(-)-????????-3-O-??-D-???????????????-4-O-??-D-???????????????

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1.????????1.1.????????????????1.1.1.????????????????????????????1000-1500??????????β???5-6???????????????????桢?????淽????????????????????????????????1?????????1mlС????-??????1??5?????????У????10???????36-37????????????????????????????????????????????????????1ml???????????????????????????????????ú???????????????80????????400????????????????????????????????????????????????4??g/ml 4??????????????????????????????6????????????????????????????????????2-3???????????????????????????????????????????????????岿???????????20-30?????????????????????????l?????????????????????????8??g/ml????????????6-15??????????????????????2С??????????????????????????????????1??10??-3??M????5-???????1??10??-3??M?????????????????????????????????????????1??10??-3??M???????????????????????????????????????????????8??g/ml??1????????忪??????????????????2???????????????????????????????????????ú?????0.5-2??g/ml????????????????????????粻????????????????????????????????????2.8??0.4??2.9??0.3??X??SE?????????????5.2??0.4??????????4??g/mL,????????????С??30???????????1.4??0.2??1.6??0.2??????????8??g/ml????????15????????????2С?????????4????????????????8??g/ml?????24??48??72??96С?????1?????????????????????????????????????3????????????3?????δ????????????????????????????????12.1??0.[FS:PAGE]6??

1.1.2.???????????????С?????????????????????????????????β??80-100????5???С????ú???300mg??kg????????75mg??kg??????????θ?????????5???????????24С?????????????????????????????????????????????????????????????????????????????????????????????????????????????????3???????????????ú?????????????????????????????12.1??0.5??13.3??0.7??????????????????????P<0.01????

1.1.3.?????????????????????????????10?????飬?????????????10??g/ml?????0.25??g/ml????????С??????????????1.5??0.5??????2.4??0.2?????????????????10??g/ml?????0.25??g/ml????????У????????????????3.3??0.2??3.1??0.2????????4.4??0.4??5.4??0.4??????????????????P<0.01????????????????????????????????????????????н????????????????????没?н??????????á????????????С??????没Ч????????????????????????????????????Ч??????????????????98-100%????????????????????????????????????????????????????????

1.2.???г?????????????1.2.1.??????β???????????????г???β?????????????????????????β???????????????????У??????16-16.5?棩?????lС???????????3-5?Σ????????????????2-3?Σ????????38???80%?????????????·??????1С??????????????????????????????á??????????????????β??286??????7?飬???38-49??????????5??1O??-4????2??10??-4????10??-4????10??-5????10??-6????10??-7?????????У????????????????·??????????????????????lС????????????87.8%??10??-7????????????????????????36.7%???????????????????????????5??10??14??????????????????????

1.2.2.??????С?г?????ú????????????????????????????????????????????10??-4?????????????????38?棻??200???160-100???50???????????10mg??????20%????????????10ml???????????????????????????????????2ml????????????38???18ml?????У????????10??-4???????????????????????????С?г棬??????????????????У???38???????·???????????????????????????к?2??5??8??10??15??20??30??40??50??60?????????????????????????????С?г?????????????????Χ???????????????β?????????С?г棬?????????100??????5?飬???20?????????????????????????????????У??????·??????????????????????10??-4??????????С?г?????5-30????????????????????????15.5??????????????????????????????????????????????????С?г档??200???160-100????50????10??-4?????????????????????????Χ????5-50??10-50??30-60????????????????????????????????????????????50??????????????????????????伸??????????????????????????90?????????????

1.2.3.??????г???????????????????????г棨Monieziaexpensa?????????г棩????38???????????15??????ù???????????????????0.3-0.4mA?????????????????????????д??????????????????????????????????????????????á???????г??????????[FS:PAGE]???38??????????????1??2??5??20??40??60????????????????????????????????1?????????????????????????38???????????У??????????????????????????????????????????????????????????????????????????á???????г?32??????6?飬??ν?????顣???????10??-4????10??-5????2??10??-6????10??-6???????????0.69??10??-5???????飨??10??-5?????????????????????2.1??10??-4?????????????????????????У???????????????·??????????????????????????????????1???????????????????忪???????2??????????????????????????????????????????????????????5??????弫??????????????????????????????????????????????????????д???????????20??????????????н???????徭???????????????????????????60?????10??-6?????????г??????????2??l0??-6????????в??????????????????????????????10??-4????10??-5?????????δ?????

1.2.4.???????г没???????????????????????????г??С??92??????????14-21????????8?飬???1?ι?θ????0.1-0.2g??kg????????????0.6-0.8g??kg?????????0.4g??kg????????0.3g??kg????0.5g??kg?????????20ml??kg????????3???????????????????????????м?顣?????????1?η???0.1g??kg???????????66.7%???????0.2g??kg??????????????С?????????г??????????????????????????????????????????????á?????????г???????????????????????????????л???ж???????????????????????

1.2.5.???????г??????????????????????????????????У?39?汣??2С???0.125mg/kg?????????????50%???????????μ????????????????????????????????????????????4-7??????????????????????????????????????Ч????????????????????????????????á???????Ч????????????????????????????????л???ж?????????????????????????????????????????????????????????????

1.2.6.???????????????????????????20?????????У????????飬????????????10??-5????10??-4????10??-3???????????38???·?????????10??-5??-10??-3??????5???????????????????10-30?????????????????????????????????????????????????????????????????????????Σ?40????????????????60???????????????????????????????????г?????????á?

2.??????????2.1.????????????????????????????6?С?????4??????????????飬?????????????????????????????????С???????????5????飬???????????3????顣????????????????????????????3.1??10??-4????6.2??10??-4??g/ml??????????5?????????С??????????????????????3.1??10??-4??g/ml?????5???????????????????????????????????6.2??10??-4??g/ml??1????????????????????????

2.2.??????????????????????????????????????????飬??????????????????????????????????????????????顣??????????????????????5??10??-4??g/ml????????????????3-7????????????????????????2.5??10??-3??g/ml?????????????1-5??????????????????

2.3.????????????????????1??????????10??????У???μ???50??????????PEG300???У?????[FS:PAGE]???????????????????5%????????????????У????????????????????????????????????????????????????????????????????????????????????????????????????????5?С????2??????2???????????????飬??????????????ú????????2-3??????????????????2-4?????顣???????1??10??-4????2??10??-4??g/ml??????????5?????????С?????????????????????3??10??-4????5??10??-4??g/ml????????1???????3????????

2.4.??????????????????????3????????????飬???????????????????????????????????????????ú??????4???????????????ú??????2??????????????????3-5?????顣???????2??10??-4????3??10??-4??g/ml????????????????5??????????????????4??10??-4????5??10??-4??g/ml????1?????????????????????????????????????????1??10??-3??g/ml??5??????????????????2??10??-3??g/ml??1??????????????????

3.???????????3.1.????????????????6-9??????????????????С???????????????????????1??10??7??-2??10??7?????????ml??????????0.5ml????????????????????????????????2.5-0.08mg??ml????????????????????37?????·?????3С??????????????0.05%?????????????????????????????????????????????????????????????????·?1С???3С???????????????????????????0.16mg??ml?????????????????????????1.25mg??ml?????1С??????????????????????????????1С?????????????7.3%??10??2??-3??10??2?????????ml????С???????????????0.2ml??????鶯?????????????????????鶯?????????????????????????飬???????????????????30mg??kg?????????????????????????????12-14?????????????????????????????????????????????S37??U14??????????????????????????????????????????47.0%??P<0.01????38.7%??P<0.05??????S180??????????????????????????????????26.3%??23.5%??P<0.05????????鶯????????????????????????????????????????鶯???????????С??????????4?????????鶯???????????????????????????????????鶯??????????????????????ü???????????С??????????????????JTC-26??????100%??HELA??????????飬??????к?????????????ó????????????????????????HELA????????γɡ???500??g/ml?????????????????????????????????????100%?????????????????????з??????????

3.2.????????????????????????????????????????????С?????S180??37??????????????????????????30mg??kg???????鶯????????????????????????5?????????鶯??????????????30mg??kg????????????????????????0.25mg?????????????????裬?????????????????????14????l5?????????????????????????????S180???????67.7%??P<0.01??????S37??????68.1%??P<0.01????????????????????Ч?????????????Ч?????

3.3.????????????????????????????????????????????1??1??2??-2??10??2?????????ml????????С????С??????0.2ml???????????????????????飬???????????30mg??kg?????1?Σ????????7?????????????????????????30????????????????????????30?????[FS:PAGE]???????????????????????????????鶯??24???????26.2??0.9??,??????25???????17.5??1.3????????????49.6%??P<0.01??????????????????????????????????????á?

3.4.??S180???????鷽???????????????????????????С????????????????????????1.5g??kg??10?????????????飬??С??S180???????????57.2%??P<0.001????53.2%??P<0.01????56.2%??P<0.01??????1000mg/kg???????????????-180???????????Σ????????????37.424%????100mg/kg????????????????-180??С??????????????????18.5%???????????7.4%???????????????????????Ｐ?????????????????á??????????????????????????????????У???????????????????????????????????????????????????????????????????????????????仵???????????????????????????????????????????????????????????????????????????????羵???????????????????????????????????????????????仯?????????????????????????????????????????????м????????????????????????????????????????????????????????????

3.5.?????????????С?????????????????????50%?????

3.6.?????С??????-37??????????á?

4.????????????????????????????????????????????????????????????о?δ???????????

4.1.?????о??????????????????????д??????????á??????????д????????????????????????????????Χ??????á?С???????????????????????45%????????????????????????????????С???????????????????????????????????????????????????Ч????

4.2.?б?????????????2mg/kg??????????????????????????????С??????????????????????飬?????????????????????С????????????????ν????????????????????20%-30%?????????????????????????δ???????????????????д???????????????(15mg/kg)????????????????????????????????????????????????????????????????á?

5.???????????????????????????????á??????????????????????????????????????????????????????????????????????????????????????????????????????????????б?????????????????????????????????????????????????????????????????????????????????á???????????????????????????????????????????????????????????????????????????????????????????????á?????????????????1??100000-1??5000????????????????????????????????????????????????????4mg???????????????????С??????????????????????е????????????????????????????????????????????????????

6.??????????????????????????????ú????????峦??????????????????????????????????????????ó??????????????????????????????????????????????λС????????????????á?

7.??????????????Ｐ???????????????????????????????????????????????????????????????????????Ч?????????????????????????????????????????????????????????????????????????????????к????????????????????Phlobaphene?????????????????????

8.??????????????????????????????[FS:PAGE]?????????????????????????????????????????????????????????????á???????????????????Ч????????????????????????????????????????????????????????????????á??????????????????????????γ没????????Ч????????????Agrimoniaeupatoria??????????????????????????????????????ù??????????????????????Ч???????????????????Ч????????????С??????????ColumbiaSK?????????á?

9.??????????????????????100mg??kg????????????ó???????????????????????????50mg/kg????Ч???????????????????????????????????????????????????????????????л???н??????á???????????е?к????????????????????????к???

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1.?????θ??С???LD50?435??88mg/kg??

2.??????????????????????????ú????????????????á???С??50?????5?顢????1?????????????????ú??????????????3????????????????С???????LD50?90.7??4.9mg??kg???????10???6??С???????鸹?????????????1?Σ?????10??????????????????????12??????????????????????????10???LD50?67.7??4.1mg??kg??

3.?????18-27g????С??210??????????????-?????????50-60????????????????м????????顣??????ù?θ?????-???????????????????θ???????????????????????50%?????5ml??kg???????????????????в???30%??????????????????3ml/kg???????3???????Karber??????????????????????????????????С???????LD50?599.3mg??kg??????????????????????LD50????540mg??kg??453.3mg??kg?????????????????????鶯?????????LD50????????P<0.01????????????????LD50????????P<0.01????С???????????????10??????????????????????????????????????????????????????????????????????Ａ??????????????1С?????????????????24С?????????

4.???????????????????θ?????????????????????????????????????????????????????????????????????????????????θ???????δ?????????????

???????1?????????20g????70%???100ml???????1h??????????????????????????????????????2ml????5%????????????2ml??????????????????????????????2ml????3??????????1ml???????????????????????? ??????2ml????5%???????2ml?????????????????????????????????2ml??????????????????3-5?Σ????????????????????2????????? ???????10g????50ml???????г?60-90?棩???????90min????????????????????????5ml??????????????????????????????????????????????????????????????????????????????轺G??????????????????????-????????-??????20??25??0.7???????????????15cm????????ɡ???????????????????????????????????????λ?????????????????