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?????????Common Threewingnut Root

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????????????????Tripterygium wilfordii Hook.f.

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????????? ???????????????????л????????????????????????????????????????????????? ????己?????????????????????2?????????????????1-2???????????????10-20cm??С?Σ???β??????2-3???????????????2cm??????100??10-6??????NAA?????1h??????????????10cm??10-15cm??壬????????60???б?????????????????????????沿??????????1/4-1/3??????????????????????????40d?????????????????1???????????????ɡ?????????????????????????50cm??50cm???????2-4??????????????磬1?1?????????6cm??????????????ɡ?

?????? ????????????1-2?Σ???6????????????????????????ù??????????????????????????Χ??????????????????????2???????????100cm???????????????????????????????1?Σ???????????100cm????????????????????????????????????????

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??????? ??????Σ????????????л??????0.5-3cm????????г????????ο顣??????????????????????????????????????????????????????????????????????????????????????????????????з???????????????????????????????????????????????????????????????????????????????????????????????????????а?????????貿???????????ζ????????д??

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?????????棺?????????????????????????????2-4?У????衣

????????????????????????????????????Σ???300-780??m?????11-28??m?????????????????????????????к??е???????[FS:PAGE]???????????6??m?????????????????????????????23-116??m????????????????????????????????????Ρ????????λ??????Σ????3-8??-17????m???????????????????Σ?????2-3??????ɡ??????????????????Ρ??????塢???????????壬???????70??m???????????????λ???Σ???????????????е????????????????????????????????Σ??е????????????????????????λ?????Σ????28-42??m??????????????????

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??????????wilfordine?????????μ?wilforine?????????????wilforgine????????????????????wil-???????celacinnine???????????-???????celafurine??????????????????celagbenzine???????????????wilforlide??A??B??????????triptonoterpenol????16-????????????????16-hydroxytripto-lide??????????????????????????triptolide?????????????????????epitriptriolide??????????????????tripterifordine???????-??????????????antriptolactone???????????tripterygic acid?????Ш????orthosphenic acid??????-?????????-sitosterol????????????daucosterol????

?????????????????????A??triptoterpenoid lactone A?????????????A??B???????????celastrol?????????????tripterine????3???22a-?????-12-?????-29-????3???22a-dihy-droxy-??12-oleanen-29-oic acid????3??24-??????-???????-29-????3??24-dioxo-friedelan-29-oic acid????3??-???-12-?????-29-????3-epikatonic acid???????????????salaspermic acid???????????????triptotriterpenic acid??A??B??C???Ш????3??? 22??-?????-12-?????-29-????3??,22??-dihydroxy-??12-oleanen-29-oic acid????2a??3a??24-?????-12-?????-28-????2a??3a??24-trihy-droxy-??12-ursene-28-oic acid????????????tripterygone???????????????????tripchlorolide???????????????????triptriolide??????????linolenic acid????8??9-?????????8??9-octadecadienoic acid????????oleic acid???????????9-hexadecenoic acid?????????palmitic acid????????stearic acid?????????????????????μ??????????????ì???????????wifornine????????????wilforjing??????????????????????triptonolide????????????????triptonide????????????????????????????????tripdiolide?????????????trip-tophenolide????????????????triptophenolide methylether???????????????neotriptophenolide??????????14-???-8??11??13-???????-3-???triptonoterpene??14-hydroxy-abieta-8??11??13-trien-3-one?????????????11-???-14-????-8??11??13-???????-3-???triptonoterpene methyl ether??11-hydroxy-14-methoxy-abieta-8??11??13-trien-3-one?????????????triptolidenol????????????hy-polide???????????????hypolide methylether???????????????isoneotriptophenolide?????????????????????????????????triptotetraolide????????????????????isotr iptetraolide????????????wilforonide?????????????A??5-?ù?-3???28-??????glut-5-en-3???28-diol????????-3???5a-??????ursan-3???5a-diol????D??A-??????-29-???????????polpunonic acid??populnonic acid??maytenonic acid????????????????triptodihy-droxy acid methyl ester????

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1.????????????????????????????????0.1mg/kg??С???????[FS:PAGE]L1210??P388?п????????????????????ED50?10-3 -10-4??g/ml???????????0.2??0.25mg/kg????????С??????L615?????????Ч???????????1??10-8mol/L,???????鰩??θ??????????MCF-7??BT-20??MKN-45??KATO-?????????????γ????????70%?????IC50?0.504-1.22??g/L??

2.??????????????????????40mg/kg??θ??????19?????????????????????????80mg/kg??θ?????????????????????????á?????????30mg/kg????????????????????????鰷??????????????????????20mg/kg ??????????????????????????????100??g/kg????????????????С??????????????????150??g/kg??????????12????5-?????????????????????????????????????0.05-1.0??g/ml??????????????????????????????????????á?

3.???????????????????????????20??40mg/kg, ????????????20??40mg/kg??θ????С??????????????????????????????С?????????????γɡ??????????75??150??g/kg????????С????岹???????????????????????IgM?????γ??200??g/kg??θ??????С????????弰??????????????????????2,4-???????????DNCB?????????????????????????????????????????0.1-1.0??g/ml,????????????ConA??PHA??PHM??LPS?????????????????????????????????????????????????á?????????1mg/kg???????С????????????????????????????????????????????0.1-1.0??g/ml????????ConA?????С?????????????????????1.0??g/ml????????????????????????10??g/ml?????????????????????????????????80mg/kg??????211mg/kg??θ?????С????????????????????????????????????????????????????????????????????????????????????????????????????????(ANAE)??????????????????????????????B????????????????????????䴙????????40??80mg/kg??????????4????????????????????????????????????????????160mg/kg????????С????????????????????????????????????????80mg/kg????????2,4-???????????DNCB????????????????????????????????????С???????????????С?????????????

4.???????????????GTW??16mg/kg??θ??????2??5?????10mg/kg???????7????????????????????????????????????????????????????????????????????-?????????????????????????????????????о??????????????????????????2??4g/kg??θ??????12 ????????????С??????????????γ??????????????á?

5.??????????????????????С???LD50?0.8mg/kg?????????LD50?0.9mg/kg??20-160??g/kg???????????7??????????????????????T??????ST??????160??g/kg???????????????????????????????????????????????????????ε?????????????????θС???LD50?1139??204??g/kg,???????1136??217??g/kg???????????????θ??С???LD50? 504.0??29.48mg/kg??

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???????? ??1?????????5g????????1??10??????????30ml??????1h???????????????????????2?????У??????????????????????0.5ml??????2ml?????????????????????1ml??????????紦????????????????????????????1ml?????1???????????????????????????????????????

??2????????????????2g??????????????У????????????????2h???????????????????????У???10g???????????????????????????????????????????????4h???????????????????1ml????ù??????????????????????0.2mg???????1ml??????????????????????????????????????????10??l??????轺G-CMC-Na?????????????-?????2??1??????????l00cm?????????2??3??5-??????????????????5??????????????????????????1??3????????????????????????????λ???????????

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1.??????練???????????????????????????????????????ɡ????4??????2500ml??????(?????)3-4С?????????250ml???????????3-4???????????θ?????練?167??(???н????27????????140??)????????2-3???????????

2.??????????????????????(????????????)5??????400ml??????2С?(?????)??????150ml?????????????100ml??????????2?η????7-10??????????????2-3??????5O??????? 1-20??????????????5-6??????????44???в???????????

3.????ν???????????β??????????????????????????????顣?31.2g???????1000ml???????尾?????????5O0ml(??10ml???0.62g)???ɡ???????3?Σ????15-20ml?????1???????????????????????????????????????????????????θ???????????10-25ml??????7-10????????????????п????????????????????????????????????????20-30?????5-7??????99??????????????????????????????????????в??????????γ??а??涨??????????????????????????????????????ж????θ???????η?????????????